Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Recombinant Proteins > Recombinant Mouse Glo1 Protein, His-tagged

Recombinant Mouse Glo1 Protein, His-tagged

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GST(1)
His(7)
Met & His(1)
N/A(1)
Cat.No.:
Glo1-62M
Product Overview:
Recombinant mouse Glo1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Description:
Glo1, also known as lactoylglutathione lyase, is a member of the glyoxalase l family. It plays a critical role in the detoxification of 2-oxoaldehydes, such as methylglyoxal. It involved in the regulation of TNF-induced transcriptional activity of NF-kappa-B. It was identified as a protein marker, which is consistently expressed to a higher extent in LAB-M than in HAB-M mice in several brain areas.
Source:
Insect cells
Species:
Mouse
Tag:
His tag
Form:
Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
Bio-activity:
Specific activity is >210 units/mg, and is defined as the amount of enzyme that will form 1.0 µmol of S-lactoylglutathione from methylglyoxal and reduced glutathione per minute at pH 6.5 at 25 centigrade.
Molecular Mass:
21.8kDa (192aa)28-40kDa (SDS-PAGE under reducing conditions)
AA Sequence:
MAEPQPASSG LTDETAFSCC SDPDPSTKDF LLQQTMLRIK DPKKSLDFYT RVLGLTLLQK LDFPAMKFSL YFLAYEDKND IPKDKSEKTAWTFSRKATLE LTHNWGTEDD ETQSYHNGNS DPRGFGHIGI AVPDVYSACK RFEELGVKFV KKPDDGKMKG LAFIQDPDGY WIEILNPNKIATIILEHHHH HH
Endotoxin:
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Purity:
>95% by SDS - PAGE
Storage:
Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20Centigrade or -70Centigrade. Avoid repeated freezing and thawing cycles.
Concentration:
0.5mg/ml (determined by Absorbance at 280nm)


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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