Active Recombinant Mouse IFNB1 Protein, Fc-tagged

• Cat.No.: IFNB1-892M
• Product Overview: Recombinant extracellular domain of mouse IFNB1 (P01575) (Met 1-Asn 182) was fused with the Fc region of human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: Fc
• Predicted N Terminal: Ile 22
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Bio-activity: Measured in antiviral assays using L929 cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is typically 10-50 pg/mL.
• Molecular Mass: The secreted recombinant mouse IFNB1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 402 amino acids and has a calculated molecular mass of 46.7 kDa. As a result of glycosylation, the apparent molecular mass of rmIFNB1/Fc monomer is approximately 58 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >92 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Ifnb1 interferon beta 1, fibroblast [ Mus musculus ]
• Official Symbol: IFNB1
• Synonyms: IFNB1; interferon beta 1, fibroblast; interferon beta; Ifb; IFNB; IFN-beta
• Gene ID: 15977
• mRNA Refseq: NM_010510
• Protein Refseq: NP_034640
• Pathway: Chagas disease (American trypanosomiasis), organism-specific biosystem; Chagas disease (American trypanosomiasis), conserved biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved
• Function: cytokine activity; cytokine receptor binding; transcription corepressor activity