Recombinant Mouse Sema7a(Gln45-Ala646) fused with Fc of Mouse IgG2A(Glu98-Lys330) at C-terminal was expressed in NS0.
Predicted N Terminal:
Lyophilized from a 0.2 μm filtered solution in PBS.
Measured by the ability of the immobilized protein to support the adhesion of THP‑1 human acute monocytic leukemia cells. Suzuki, K. et al. (2007) Nature 446:680. The ED50 for this effect is typically 0.1-0.5 μg/mL.
Predicted Molecular Mass: 95.5 (monomer) kDaSDS-PAGE: 100-110 kDa, reducing conditions
<0.10 EU per 1 μg of the protein by the LAL method.
>85%, by SDS-PAGE under reducing conditions and visualized by silver stain
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.