Recombinant Mouse TNFSF11 Protein, Fc-tagged
Recombinant extracellular domain of mouse TNFSF11 (BAA97257.1) (Arg 72-Asp 316) was fused with the Fc region of human IgG1 at the N-terminus.
Predicted N Terminal:
Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Immobilized mouse Fc-TNFSF11 at 10 μg/ml (100 μl/well) can bind biotinylated human TNFRSF11B-His, The EC50 of biotinylated human TNFRSF11B-His is 0.07-0.17 μg/ml.
The recombinant mouse TNFSF11/Fc is a disulfide-linked homodimer. The reduced monomer comprises 505 amino acids and has a calculated molecular mass of 56 kDa. The apparent molecular mass of the monomer is approximately 50 kDa in SDS-PAGE under reducing conditions.
< 1.0 EU per μg of the protein as determined by the LAL method.
>87 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.