Active Recombinant Murine Wnt3a protein

• Cat.No.: Wnt3a-526M
• Product Overview: Recombinant Murine Wnt3a(Ser19-Lys352) was expressed in CHO.

Specification

• Description: Protein Wnt-3a is a protein that is encoded by the WNT3A gene. The WNT gene family consists of structurally related genes that encodesecreted signaling proteins. These proteins have been implicated in oncogenesis, adipogenesis, etc. and in several other developmentalprocesses, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family.Mouse Wnt3a shows 96% amino acid identity to human Wnt3a protein.
• Source: CHO
• Species: Murine
• Form: Tris-HCl buffer pH 7.2-7.4, 0.5% CHAPS, 0.1% BSA.
• Bio-activity: Wnt3a activity has been measured using TCF-based Wnt reporter stable cell line (NIH3T3). 4 ng/mL of Wnt3a generate twenty fold increase of luciferase activity compared to control (buffer without Wnt3a).
• Molecular Mass: Predicted M.W.: 37 kDa;Actual M.W.: 41 kDa evaluated by SDS-PAGE under reducing conditions
• Purity: >75% evaluated by SDS-PAGE under reducing conditions
• Storage: This preparation can be stored at 4 centigrade for 6 months; Storage under frozen conditions may decrease activity; avoid freeze-thaw.
• Concentration: 4 µg/mL

Gene Information

• Gene Name: Wnt3a wingless-type MMTV integration site family, member 3A [ Mus musculus ]
• Official Symbol: Wnt3a
• Synonyms: vt; Wnt-3a; vestigial tail; wingless-related MMTV integration site 3A
• Gene ID: 22416
• mRNA Refseq: NM_009522
• Protein Refseq: NP_033548
• UniProt ID: P27467
• Chromosome Location: 11 B1.3; 11 37.17 Cm
• Pathway: Basal cell carcinoma, organism-specific biosystem; Class B/2 (Secretin family receptors), organism-specific biosystem; Melanogenesis, organism-specific biosystem
• Function: frizzled binding; protein binding; receptor agonist activity