Recombinant rat CST3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
CST3, also known as cystain-C, is secreted type 2 cysteine proteinase inhibitors of the cystatin superfamily. It is involved in processes such as tumor invasion and metastasis, inflammation and some neurological diseases. It inhibits many cysteine proteases such as papain and cathepsins B, H, K, L and S.
Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
14.3kDa (128aa)13.5-18KDa (SDS-PAGE under reducing conditions.)
GTSRPPPRLL GAPQEADASE EGVQRALDFA VSEYNKGSND AYHSRAIQVV RARKQLVAGI NYYLDVEMGR TTCTKSQTNL TNCPFHDQPHLMRKALCSFQ IYSVPWKGTH TLTKSSCKNA LEHHHHHH
< 1.0 EU per 1 microgram of protein (determined by LAL method)
>95% by SDS - PAGE
Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20Centigrade or -70Centigrade. Avoid repeated freezing and thawing cycles.
0.5mg/ml (determined by Absorbance at 280nm)