Recombinant Rat Fgf23(aa 25-251) fused with His tag was expressed in E. coli.
FGF23 is a member of the fibroblast growth factor family of proteins, which possess broad mitogenic and cell survival activities and are involved in a variety of biological processes. The product of this gene regulates phosphate homeostasis and transport in the kidney. The full-length, functional protein may be deactivated via cleavage into N-terminal and C-terminal chains. Mutation of this cleavage site causes autosomal dominant hypophosphatemic rickets (ADHR).
20 mM Tris, pH 8.0, 500 mM NaCl, 50% glycerol
YSDTSPLLGS NWGSLTHLYT ATARNSYHLQ IHRDGHVDGTPHQTIYSALM ITSEDAGSVV IIGAMTRRFL CMDLRGNIFGSYHFSPENCR FRQWTLENGY DVYLSPKHHY LVSLGRSKRIFQPGTNPPPF SQFLARRNEV PLLHFYTARP RRHTRSAEDPPERDPLNVLK PRPRATPIPV SCSRELPSAE EGGPAASDPLGVLRRGRGDA RRGAGGTDRC RPFPRFV
Long term: -80 centigrade; Short term: -20 centigrade. Avoid freeze-thaw cycles.