Active Recombinant Rat LDHA protein(Met1-Phe332), His-tagged

• Cat.No.: Ldha-1131R
• Product Overview: Recombinant Rat Ldha (NP_058721.1) (Met1-Phe332) was expressed in E. coli with a polyhistidine tag at the N-terminus.

Specification

• Source: E. coli
• Species: Rat
• Tag: N-His
• Protein length: Met1-Phe332
• Form: Lyophilized from sterile 50 mM Tris, 50 mM NaCl. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to reduce pyruvate to lactate. The specific activity is >50,000 pmoles/min/μg.
• Molecular Mass: The recombinant Rat Ldha consists 350 amino acids and predicts a molecular mass of 38.7 kDa.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Reviews

02/28/2023

The protein composition was stable and concentrated, allowing me to obtain satisfactory experimental results.

01/25/2022

The LDHA proteins purchased from your company is suitable for ELISA.

01/07/2022

The technician modified my experimental protocol so that my experiment went smoothly.

Q&As

What diseases has LDHA been studied in relation to?

LDHA has been associated with studies of diseases such as breast, stomach and lung adenocarcinomas.

Where is LDHA located?

LDHA is mainly located in the cytoplasm.

How does LDHA affect the development of gastric cancer?

LDHA inhibits the growth and invasion of gastric cancer cells and promotes apoptosis by inhibiting glycolysis.

What is the expression level of LDHA in breast cancer tissues?

LDHA expression levels are upregulated in breast cancer tissues.

How is LDHA related to nicotine?

LDHA binds directly to nicotine and is a target for nicotine.