Recombinant Rat Rtn4 protein, Fc-tagged
Recombinant Rat Rtn4(Thr544 - Phe725) fused with Human IgG1(Pro100 - Lys330) at C-terminal was expressed in NS0.
Predicted N Terminal:
Lyophilized from a 0.2 μm filtered solution in PBS.
Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly inhibit neurite outgrowth when immobilized as a 3 µL droplet containing 300 ng on a nitrocellulose-coated microplate.
Predicted Molecular Mass: 46.1 (monomer) kDa;SDS-PAGE: 75-85 kDa, reducing conditions.
<0.10 EU per 1 μg of the protein by the LAL method.
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitute at 100 μg/mL in sterile PBS.