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Home > Products > Recombinant Proteins > Recombinant Zika virus flavivirus polyprotein gene protein, His & Avi-tagged, Biotinylated

Recombinant Zika virus flavivirus polyprotein gene protein, His & Avi-tagged, Biotinylated

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By Species
By Tag
GST(1)
His(11)
His & Avi(1)
Cat.No.:
flavivirus polyprotein gene-1824Z
Product Overview:
Biotinylated Zika virus NS1, His Tag is expressed from human 293 cells (HEK293). It contains AA Val 796 - Ser 1148 (Accession # ALU33341.1). Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
Description:
Zika virus (ZIKV) is a member of the virus family Flaviviridae and the genus Flavivirus, transmitted by daytime-active Aedes mosquitoes, such as A. aegypti and A. albopictus. Its name comes from the Zika Forest of Uganda, where the virus was first isolated in 1947. Zika virus is related to dengue, yellow fever, Japanese encephalitis, and West Nile viruses. The infection, known as Zika fever, often causes no or only mild symptoms, similar to a mild form of dengue fever. It is treated by rest. Since the 1950s, it has been known to occur within a narrow equatorial belt from Africa to Asia. As of 2016, the illness cannot be prevented by drugs or vaccines. As of February 2016, there is evidence that Zika fever in pregnant women is associated with abnormal brain development in their fetuses through mother-to-child transmission of the virus, which may result in miscarriage or microcephaly.
Source:
HEK293
Species:
Zika virus
Tag :
His & Avi
Predicted N Terminal:
Val 796
Form:
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass:
This protein carries an Avi tag at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 43.3 kDa. The protein migrates as 46-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length:
AA Val 796 - Ser 1148
Endotoxin:
Less than 1.0 EU per μg by the LAL method.
Purity:
>90% as determined by SDS-PAGE.
Storage:
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution:
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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