The TAF1L (BD1+BD2) TR-FRET Assay Kit is designed to measure the inhibition of TAF1L (BD1+BD2) binding to its substrate in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, TAF1L (BD1+BD2), substrate, and an inhibitor is incubated for 120 minutes. Then, the fluorescence intensity is measured using a fluorescence reader.
Great for screening small molecular inhibitors for drug discovery and HTS applications.
At least 6 months from date of receipt when stored as directed.
TAF1L (BD1+BD2), GST-tag: 10 µg; -80°CBET Bromodomain Ligand: 50 µl; -80°C Non-acetylated Ligand 1: 15 µl; -20°C Tb-labeled donor: 20 µl; -20°C Dye-labeled acceptor: 20 µl; -20°C 3x BRD TR-FRET Assay Buffer 1: 4 ml; -20°C White, Nonbinding Corning, low volume, microtiter plate: 1; Room temp.