Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Activity Kits > TNKS1 Histone Ribosylation Colorimetric Assay Kit

TNKS1 Histone Ribosylation Colorimetric Assay Kit

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Cat.No.:
Kit-1883
Size:
96 reactions
Description:
The TNKS1 Histone Ribosylation Colorimetric Assay Kit is designed to measure Tankyrase 1 (TNKS1) activity for screening and profiling applications. TNKS1 catalyzes the NAD-dependent addition of poly(ADP-ribose) to the substrate proteins. The key to the TNKS1 Histone Ribosylation Assay is the biotinylated NAD+ substrate. With this kit, only three simple steps are required for TNKS1 reactions. First, histone proteins are coated on a 96-well plate. Next, the biotinylated NAD+substrate is incubated with an assay buffer that contains the TNKS1 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the colorimetric HRP substrate to produce color that can then be measured using a UV/Vis spectrophotometer microplate reader.
Applications:
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Storage:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Kit Components:
TNKS1: 5 µg; -80°C 5 x histone mixture: 1 ml; -80°C10x Assay Mixture Containing Biotinylated Substrate: 300 μl; -80°C10x PARP Assay Buffer: 1 ml; -80°CBlocking buffer: 25 ml; +4°CStreptavidin-HRP: 100 μ; +4°CColorimetric HRP substrate: 10 ml; +4°C Transparent 96-well plate: 1; Room Temperature


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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