Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email:
Home > Products > Assay Kits > Activity Kits > TNKS2 Histone Ribosylation Assay Kit (Antibody Detection) I

TNKS2 Histone Ribosylation Assay Kit (Antibody Detection) I

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The Tankyrase-2 Histone Ribosylation Assay Kit (Antibody Detection) is designed to measure Tankyrase-2 activity for screening and profiling applications. The key to the Tankyrase-2 Histone Ribosylation Assay Kit is a highly specific antibody that recognizes poly (ADP-ribose) modified histone. With this kit, only three simple steps on a microtiter plate are required for Tankyrase-2 reaction. First, NAD+ is incubated with a sample containing assay buffer and enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Kit Components:
TNKS2: 2 µg; -80°C 5 x histone mixture: 1 ml; -80°CNAD+ : 250 µl; -80°C10x PARP Assay Buffer: 1 ml; -80°CPrimary antibody 11: 13 µl; -80°C Secondary HRP-labeled antibody 1: 10 µl; -80°CBlocking buffer: 80 ml; +4°C HRP chemiluminescent substrate A (2 components): 6 ml each; +4°C 8-well strip plate module: 1 plate (12 x 8-well strips); +4°C

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

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