Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Cellular Kits > TORC2 Nuclear Translocation Assay Kit

TORC2 Nuclear Translocation Assay Kit

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Cat.No.:
Kit-1673
Product Overview:
Nuclear Translocation cells are engineered to co-two fusion proteins: a) Enzyme Donor (ED) tagged target protein; b) an Enzyme Acceptor (EA) tagged TAZ domain, derived from the CBP/P300 transcription factor, that localizes to the nucleus. Depending on the assay, activation of the signaling pathway can either a) induce translocation of the ED-tagged target protein into the nucleus, which will force complementation of the two enzyme fragments, and result in the formation of a functional enzyme that will hydrolyze substrate and generate a chemiluminescent signal; or b) induce the ED-tagged protein to vacate the nucleus, resulting in a decrease of functional enzyme and a subsequent decrease of chemiluminescent signal. Some nuclear translocation assays will also co-an untagged secondary protein involved in the pathway of interest. These cells have been modified to prevent long term propagation and expansion using a proprietary compound that has no apparent effect on assay performance.
Size:
100 dp(1 x 96-well)/200 dp(2 x 96-well)/1000 dp(10 x 96-well)
Target Species:
Human
Storage:
Short term (2 weeks): Store in vapor phase of liquid N2
Kit Components:
1 x 10^6 cells per vial in 100uL mL (kit contains cells, detection reagents and plate(s))


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Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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