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Transglutaminase Activity Colorimetric Assay Kit

Cat.No.: Kit-0814
Product Overview: Simple, rapid & convenientThe assay can detect transglutaminase activity ~10 µU or 80 ng of recombinant hTG2 enzyme
Size: 100 assays
Description: Transglutaminases (EC 2.3.2.13) are calcium dependent enzymes that catalyze the post-translational modification of proteins by formation of isopeptide bonds. This occurs either through protein cross-linking via formation of γ-glutamyl-ε-lysine bonds or through incorporation of primary amines at selected peptide-bound glutamine residues. The transglutaminase enzyme family comprises the intracellular forms (TG1, TG3 and TG5) expressed mostly in the epithelial tissue; TG2 which is both intracellular and extracellular and expressed in various tissue types; TG4 which is expressed in the prostate gland; factor XIII which is expressed in blood; TG6 and TG7, whose tissue distribution is unknown and band 4.2 (lacking enzymatic activity) which is present on erythrocyte membranes. Transglutaminases also exhibit GTPase, phosphodiesterase and protein kinase activity. Transglutaminases are associated with certain neurological and autoimmune disorders and also cancer. Transglutaminase Activity Assay kit utilizes the deamidation reaction of the transglutaminase enzyme with a donor and acceptor substrate resulting in the formation of a hydroxamate product. The hydroxamate product reacts with the Stop Solution forming a purple complex that can be measured colorimetrically at 525 nm. The limit of quantification of this assay is ~10 µU or 80 ng of recombinant hTG2 enzyme.
Applications: Measurement of Transglutaminase activity
Usage: For Research Use Only! Not For Use in Humans.
Storage: -20°C
Kit Components: • TG Assay Buffer• Homogenization Buffer (10x)• 1M DTT• Donor Substrate • Acceptor Substrate • Hydroxamate Standard • Stop Solution • Positive Control• Plate Sealer
Detection method: Absorbance (525 nm)
Compatible Sample Types: • Cell lysate• Tissue lysate• Recombinant Transglutaminase

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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