Ac-IETD-AFC
| Cat.No. : | DYE-126 |
- Specification
- Gene Information
- Related Products
- Download
| Description : | Ac-IETD-AFC is a fluorogenic substrate that can be cleaved by caspase-8 and related enzymes that recognize the amino acid sequence IETD, including caspase-3 processing enzyme, caspase-10, and granzyme B. Caspase activity can be quantified by fluorescent detection of free AFC (also known as 7-amino-4-trifluoromethylcoumarin), which is excited at 400 nm and emits at 505 nm. |
| Molecular Mass : | 729.65 |
| Ex(nm) : | 380 |
| Em(nm) : | 500 |
| Solvent : | DMSO |
| Storage : | Store at -20°C in the dark. Dessicated. |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
- Q&As
- Reviews
Q&As (6)
Ask a questionThe Ac-IETD-AFC assay can be used for high-throughput screening due to its sensitivity and simplicity, making it a useful tool for drug discovery and other applications where caspase-8 activity needs to be measured in large numbers of samples.
The principle behind the Ac-IETD-AFC assay is based on the detection of caspase-8 activity through the cleavage of the substrate Ac-IETD-AFC, which releases a fluorescent compound that can be measured.
7. EDTA is not recommended for use in sample collection for the Ac-IETD-AFC assay due to potential metal-ion interference with the assay. Other anticoagulants like heparin or citrate are recommended instead.
The Ac-IETD-AFC assay is specific for caspase-8 activity and does not detect other compounds.
The Ac-IETD-AFC assay kit is typically used with cell lysates or tissue homogenates, but can also be used with purified protein samples.
Ac-IETD-AFC stands for N-acetyl-Ile-Glu-Thr-Asp-7-amido-4-trifluoromethylcoumarin.
Customer Reviews (3)
Write a reviewA satisfactory shopping experience!
Like to buy products from this company, the after-sales service is very comprehensive.
Same quality as others but more affordable price.
Ask a Question for All Ac-IETD-AFC Products
Required fields are marked with *
My Review for All Ac-IETD-AFC Products
Required fields are marked with *
Inquiry Basket
