Acitve Recombinant Rat EphA7 protein(Met1-Ser539), His-tagged

• Cat.No.: EphA7-1255R
• Product Overview: Recombinant Rat EPHA7 (Met1-Ser539) was expressed in HEK293 with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Rat
• Tag: C-His
• Protein length: Met1-Ser539
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized Rat EPHA7-His at 10 μg/ml (100 μl/well) can bind Mouse EFNA4-Fc, The EC50 of Mouse EFNA4-Fc is 10.0-23.4 ng/ml.
• Molecular Mass: The recombinant Rat EPHA7 comprises 523 amino acids and predicts a molecular mass of 58.9 kDa. The apparent molecular mass of the recombinant protein is approximately 59 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Epha7 Eph receptor A7 [ Rattus norvegicus ]
• Official Symbol: EphA7
• Synonyms: EPHA7; Eph receptor A7; ephrin type-A receptor 7; EHK-3; EPH homology kinase 3; ephrin receptor EphA7; tyrosine-protein kinase receptor EHK-3
• Gene ID: 171287
• mRNA Refseq: NM_134331
• Protein Refseq: NP_599158