| Description : |
Dickkopf-associated protein 1 (Dkk-1) is the initial member of the Dickkopf protein family, which includes Dkk-1, Dkk-2, Dkk-3, Dkk-4 and the related protein Soggy. dkk protein is a secreted protein with two conserved cysteine-rich structural domains. Each structural domain contains 10 cysteine residues. Human mature Dkk-1 is a glycosylated protein with a molecular weight of 40 KD. Dkk-1 acts as an antagonist of Wnt signaling, and Dkk-1 is expressed throughout development and antagonizes Wnt-7a during limb development. The balance between Wnt signaling and Dkk-1 inhibition is critical for bone formation and internal environmental stability. Insufficient or excessive Dkk-1 activity in bone leads to increased or decreased BMD, respectively. In adults, Dkk-1 is expressed in osteoblasts, osteocytes and neurons. Brain ischemia induces Dkk-1 expression, leading to neuronal death. |
| Source : |
CHO-K1 |
| Species : |
Human |
| Form : |
Lyophilized powder |
| Endotoxin : |
<20 EU/mg |
| Bio-activity : |
>5.0 x 10^4 IU/mg |
| Purity : |
>95.0% by high performance liquid chromatography (SEC-HPLC) and SDS-PAGE. |
| Storage Buffer : |
10mM Phosphate-Buffered Saline (pH 7.2) |
| Storage : |
Stable storage at 4℃ for 7-10 days; for long-term storage, please store at -20℃ after dispensing. |
| Sterility : |
Sterility |
| Instructions : |
It is recommended that lyophilized GMP rhDKK1 be dissolved in water for injection or sterilized ultrapure water at a concentration of not less than 100 μg/ml, pending further dilution to working concentration. |
