Active GMP Recombinant Human RSPO1 Protein
Cat.No. : | RSPO1-01HG |
Product Overview : | Recombinant human RSPO1 protein, was expressed in CHO-K1 cell-derived in an animal component free process under cGMP guidelines. |
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Description : | This gene encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. Alternative splicing results in multiple transcript variants. |
Source : | CHO-K1 |
Species : | Human |
Form : | Lyophilized powder |
Endotoxin : | <20 EU/mg |
Bio-activity : | >5.0 x 10^5 IU/mg |
Purity : | >95.0% by high performance liquid chromatography (SEC-HPLC) and SDS-PAGE. |
Storage Buffer : | 10mM Phosphate-Buffered Saline (pH 7.2) |
Storage : | Stable storage at 4℃ for 7-10 days; for long-term storage, please store at -20℃ after dispensing. |
Sterility : | Sterility |
Instructions : | It is recommended that lyophilized GMP rhRSPO1 be dissolved in water for injection or sterilized ultrapure water at a concentration of not less than 100 μg/ml, pending further dilution to working concentration. |
Gene Name : | RSPO1 R-spondin 1 [ Homo sapiens (human) ] |
Official Symbol : | RSPO1 |
Synonyms : | RSPO; CRISTIN3 |
Gene ID : | 284654 |
mRNA Refseq : | NM_001038633.4 |
Protein Refseq : | NP_001033722.1 |
UniProt ID : | Q2MKA7 |
MIM : | 609595 |
Products Types
◆ Recombinant Protein | ||
RSPO1-34H | Recombinant Human RSPO1 Protein | +Inquiry |
RSPO1-422H | Recombinant Human RSPO1 Protein, His-tagged | +Inquiry |
Rspo1-8672M | Active Recombinant Mouse Rspo1 protein(Met1-Gln265), His-tagged | +Inquiry |
RSPO1-28H | Active Recombinant Human RSPO1 Protein, Pre-aliquoted | +Inquiry |
RSPO1-278H | Recombinant Active Human RSPO1 Protein, His-tagged(C-ter) | +Inquiry |
◆ Lysates | ||
RSPO1-001MCL | Recombinant Mouse RSPO1 cell lysate | +Inquiry |
RSPO1-001HCL | Recombinant Human RSPO1 cell lysate | +Inquiry |
RSPO1-1918HCL | Recombinant Human RSPO1 cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionRSPO1 stands out for its potential therapeutic use in Regenerative Medicine due to its mitogenic activity in stem cells.
Characterization methods included Western blotting, mass spectrometry, and in vitro (C2C12 cells) and in vivo (BALB/c mice) biological activity assays, confirming structural integrity and biological efficacy.
RSPOs regulate various tissue-specific processes, including bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells, intestinal stem cells, and cancer progression.
The purification strategy for rhRSPO1 involved two sequential chromatographic steps: heparin affinity chromatography followed by molecular exclusion chromatography.
RSPO1 induces the Wnt pathway by stabilizing the Wnt receptor complex, leading to enhanced Wnt signaling and influencing cellular processes.
rhRSPO1 was generated using the HEK293 cell line, and the coding sequence was synthesized and subcloned into a mammalian cell expression vector.
R-Spondin proteins, including RSPO1, play crucial roles in cell proliferation, differentiation, and death by inducing the Wnt pathway.
Customer Reviews (3)
Write a reviewRemarkable changes in cell behavior were attributed to the potent effects of the product.
The product's respected reputation in the scientific community was a testament to its quality and effectiveness.
Dependable protein-protein interaction outcomes were achieved due to the product's pure composition.
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