Active GMP Recombinant Mouse Nrtn Protein, His-Tagged

• Cat.No.: Nrtn-01M
• Product Overview: GMP Recombinant Mouse Nrtn Protein, His-Tagged was expressed in Escherichia coli cell-derived in an animal component free process under cGMP guidelines.

Specification

• Description: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. The encoded preproprotein is proteolytically processed to generate the mature protein. This protein signals through the RET receptor tyrosine kinase and a GPI-linked coreceptor, and promotes survival of neuronal populations. Homozygous knockout mice for this gene exhibit defects in the development of the retina and enteric nervous system, and reduced cholinergic innervation of the heart and lacrimal glands.
• Source: Escherichia coli
• Species: Mouse
• Tag: His
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce proliferation in SH-SY5Y cells. The ED50 for this effect is <50 ng/mL.
• AA Sequence: PGARPCGLRELEVRVSELGLGYTSD ETVLFRYCAGACEAAIRIYDLGLRR LRQRRRVRRERARAHPCCRPTAYED EVSFLDVHSRYHTLQELSARECACV with polyhistidine tag at the N-terminus
• Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE. Ni-NTA chromatography
• Notes: Please use within one month after protein reconstitution.
• Storage: Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.
• Storage Buffer: The protein was lyophilized from a solution containing 1X PBS, pH 7.4.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

Gene Information

• Gene Name: Nrtn neurturin [ Mus musculus (house mouse) ]
• Official Symbol: Nrtn
• Synonyms: NTN
• Gene ID: 18188
• mRNA Refseq: NM_008738.3
• Protein Refseq: NP_032764.1
• UniProt ID: P97463