Active Native Bacillus stearothermophilus Acetate Kinase

• Cat.No.: Acetate Kinase-22B
• Product Overview: Native Bacillus stearothermophilus Acetate Kinase was purified from Bacillus stearothermophilus.

Specification

• Description: Acetate kinase plays an important role in glycolysis. Acetate kinase phosphorylates acetate in the presence of ATP and a divalent cation, which ultimately results in the production of acetyl-CoA. Acetate kinase is also involved in the metabolism of propanoate, pyruvate and taurine. Acetate Kinase from Bacillus stearothermophilus is a thermostable tetramer of identical subunits with molecular weight of 40,000 Da each. The enzyme does not have a -SH group and is composed of 36% β-structure, 21 α-helix and 43 % unordered structure.
• Source: Bacillus stearothermophilus
• Species: Bacillus stearothermophilus
• Form: Lyophilized powder containing potassium phosphate.
• Bio-activity: Involved in the metabolism of propanoate, pyruvate and taurine. 400-1,200 units/mg solid.
• Unit Definition: One unit will phosphorylate 1.0 μmole of acetate to acetyl phosphate per min at pH 7.2 at 30 °C.
• Applications: Acetate kinase is used to phosphorylate acetate to acetyl phosphate. Acetate Kinase from Bacillus stearothermophilus has been used to study allosteric activation. [32P]-acetyl phosphate was generated by incubating potassium acetate in the reaction mixture with acetate kinase from Sigma. This [32P]-acetyl phosphate was used to label BldM, BldM D-54N or BldM D-54A loci during the study of the effect of bldM gene on Streptomyces coelicolor development.
• Storage: 2-8°C.

Reviews

03/16/2022

The purchase process is pleasant and easy to operate!

09/22/2020

High purity. high activity.

04/16/2019

It is the second time to buy your product.

Q&As

Does acetate kinase exist only in archaea?

In recent years, ACK has also been identified in certain eukaryotic microbes including the green algae Chlamydomonas, euascomycete and basidiomycete fungi, and certain protists, namely Entamoeba histolytica.

Why is it said that acetate kinase plays an important role in glycolysis?

Acetate kinase phosphorylates acetate in the presence of ATP and divalent cations, resulting in the production of acetyl-CoA.

Is acetate kinase in monomeric or dimeric form?

It exists as a dimer with a nucleotide binding site in the gap between the N- and C-terminal domains.

Can magnesium ions act as a cofactor for acetate kinase?

Yes. The cofactors of acetate kinase are Mg2+ and Mn2+.

What is the function of acetate kinase?

Acetate kinase catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction. During anaerobic growth of the organism, this enzyme is also involved in the synthesis of most of the ATP formed catabolically.

How many acetate kinase isoenzymes are there in L lactis?

There are two acetate kinase isozymes in L lactis.

Acetate kinase is mainly found in which organisms?

Acetate kinase is widely found both in anaerobic and aerobic microbes of the Bacteria and Archaea domains and plays a central role in major links of the global carbon cycle.

Is acetate kinase a phosphotransferase?

yes, it is. Acetate kinaseone is one of the earliest phosphoryltransfer enzymes to be recognized.