Source : |
HEK293 |
Species : |
Bovine |
Tag : |
His-Avi |
Predicted N Terminal : |
Ala 24 (FCGRT) & Ile 21 (B2M) |
Form : |
Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose. |
Bio-activity : |
Immobilized Ipilumimab at 2 ug/mL (100 uL/well) can bind Biotinylated Bovine FcRn Heterodimer Protein with a linear range of 2-20 ug/mL |
Molecular Mass : |
The protein has a calculated MW of 34.5 kDa (FCGRT), 11.6 kDa (B2M). The protein migrates as 37 kDa (FCGRT), 12 kDa (B2M) respectively on a SDS-PAGE gel under reducing condition due to glycosylation. |
Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method. |
Purity : |
>90% as determined by SDS-PAGE. |
Characteristic : |
Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay. |
Stability : |
Samples are stable for up to twelve months from date of receipt at -70ºC. |
Storage : |
For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
Official Symbol : |
FCGRT; B2M |
Synonyms : |
FcRn, FCGRT & B2M |