Active Recombinant Bovine TMPRSS15 Protein (200 aa), His-tagged

• Cat.No.: TMPRSS15-158T
• Product Overview: Recombinant Bovine Enterokinase (rbEK) as the light chain is a single glycosylated polypeptide chain containing 200 amino acids. A fully biologically active molecule, rbEK has a molecular mass of 22.7 kDa and is obtained by proprietary chromatographic techniques.

Specification

• Description: Enterokinase (EK) is an enzyme produced by cells of the duodenum and involved in human digestion. It plays a role of turning trypsinogen to its active form trypsin, and indirectly activates the pancreatic digestive enzymes. Enterokinase is a specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys. Enterokinase will not work if the recognition site is followed by a proline.
• Source: P. pastoris
• Species: Bovine
• Tag: His
• Form: Sterile Filtered White lyophilized (freeze-dried) powder.
• Bio-activity: 5 IU/μL
• Molecular Mass: Theoretical MW: 22.7 kDa.Apparent MW: 40.0 kDa, observed by reducing SDS-PAGE.
• Protein Length: 200
• Endotoxin: < 1 EU/μg, determined by LAL method.
• Purity: > 95% by SDS-PAGE analyses.
• Unit Definition: One unit is defined as the amount of enzyme needed to cleave 50 μg of fusion protein in 16 hours to 95% completion at 22 centigrade in a buffer containing 25mM Tris-HCl, pH 8.0.
• Storage: Recombinant Bovine Enterokinase (rbEK) remains stable up to 1 year at -20 centigrade from date of receipt. It will remain stable at 37 centigrade for one week without losing any activity.Please avoid freeze-thaw cycles.
• Storage Buffer: Sterile liquid solution contians 20mM Tris, 200mM NaCl, 2mM CaCl2, 50% glycerol, pH 7.4.
• Reconstitution: Reconstituted in ddH2O or PBS at 100 μg/mL.

Gene Information

• Gene Name: TMPRSS15 transmembrane serine protease 15 [ Bos taurus (cattle) ]
• Official Symbol: TMPRSS15
• Synonyms: TMPRSS15; transmembrane serine protease 15; PRSS7; enteropeptidase; enterokinase; protease, serine, 7 (enterokinase); serine protease 7; transmembrane protease, serine 15; EC 3.4.21.9
• Gene ID: 282009
• mRNA Refseq: NM_174439
• Protein Refseq: NP_776864
• UniProt ID: P98072