Active Recombinant Canine PDCD1 protein, His-Avi-tagged, Biotinylated
Cat.No. : | PDCD1-0610C |
Product Overview : | Biotinylated Recombinant Canine PDCD1 protein(Leu25-Gly168), fused with His and Avi tag, was expressed in HEK293. |
- Specification
- Gene Information
- Related Products
Source : | HEK293 |
Species : | Canine |
Tag : | His-Avi |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : | Immobilized Human PD-L1, Fc Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Canine PD-1, His,Avitag with a linear range of 0.001-0.125 μg/mL. Immobilized Biotinylated Canine PD-1, His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Monoclonal Anti-Human PD-1 Antibody, Human IgG4 with a linear range of 0.004-1 μg/mL. |
Molecular Mass : | This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag).The protein has a calculated MW of 19.7 kDa. The protein migrates as 32-36 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Proteinlength : | Leu25-Gly168 |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >90% as determined by SDS-PAGE. |
Storage : | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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