Active Recombinant Cynomolgus ACVR2B protein(Ser28-Thr143), His-tagged

• Cat.No.: ACVR2B-3386C
• Product Overview: Recombinant Cynomolgus ACVR2B (Ser28-Thr143) was expressed in HEK293 with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Cynomolgus
• Tag: C-His
• Protein length: Ser28-Thr143
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 0.2-1 μg/mL in the presence of 10 ng/mL recombinant Activin A.
• Molecular Mass: The recombinant cynomolgus ACVR2B comprises 127 amino acids and has a calculated molecular mass of 14.8 KDa. The apparent molecular mass of it is approximately 33-36 KDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.