Active Recombinant Cynomolgus FCGR2B protein, His-Avi-tagged, Biotinylated

• Cat.No.: FCGR2B-1440C
• Product Overview: Biotinylated Recombinant Cynomolgus FCGR2B protein(Ala46-Pro224), fused with His and Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Cynomolgus
• Tag: His-Avi
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.
• Bio-activity: Loaded Biotinylated Cynomolgus CD32b, His,Avitag on SA Biosensor, can bind MabThera (Rituximab) with an affinity constant of 1.3 μM as determined in BLI assay (ForteBio Octet Red96e).
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).The protein has a calculated MW of 23.6 kDa. The protein migrates as 30-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Ala46-Pro224
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.