Active Recombinant Cynomolgus Monkey Klb Protein (Phe53-Leu997), C-6×His tagged

• Cat.No.: Klb-34M
• Product Overview: Recombinant Cynomolgus Monkey Klotho beta His Protein (Phe53-Leu997) with a C-terminal 6-His tag was expressed in Mouse myeloma cell line.

Specification

• Description: Predicted to enable fibroblast growth factor binding activity and fibroblast growth factor receptor binding activity. Acts upstream of or within positive regulation of MAPKKK cascade by fibroblast growth factor receptor signaling pathway and positive regulation of cell population proliferation. Predicted to be integral component of membrane. Is expressed in several structures, including extraembryonic component; gut; liver; lung; and trunk mesenchyme. Orthologous to human KLB (klotho beta).
• Source: Mouse myeloma cell line
• Species: Monkey
• Tag: C-6×His
• Bio-activity: Measured by its binding ability in a functional ELISA.
  When Recombinant Human FGF-21 is immobilized at 1 μg/mL (100 μL/well), Recombinant Cynomolgus Monkey Klotho beta His-tag binds with an ED50 of 0.05-0.4 μg/mL.
• Molecular Mass: Predicted Molecular Mass: 110 kDa
  SDS-PAGE: 110-130 kDa, under reducing conditions
• Protein Length: Phe53-Leu997
• N-terminal Sequence Analysis: Phe53
• Endotoxin: < 0.10/μg of the protein by the LAL method.
• Purity: >90% by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -70 centigrade as supplied.
  3 months, -70 centigrade under sterile conditions after opening.
  1 month, 2 to 8 centigrade under sterile conditions after opening.
• Storage Buffer: Supplied as a 0.2 μm filtered solution in PBS, Glycerol and EDTA.
• Shipping: The product is shipped with dry ice or equivalent.

Gene Information

• Gene Name: Klb klotho beta [ Mus musculus (house mouse) ]
• Official Symbol: Klb
• Synonyms: Klb; klotho beta; betaKlotho; beta-klotho; BKL; klotho beta-like protein
• Gene ID: 83379
• mRNA Refseq: NM_031180
• Protein Refseq: NP_112457
• UniProt ID: Q920J2