Source : |
HEK293 |
Species : |
Cynomolgus |
Tag : |
lFc |
Form : |
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally Trehalose is added as protectant before lyophilization. |
Bio-activity : |
Immobilized Cynomolgus NKp46, Llama IgG2b Fc Tag at 1 μg/mL (100 μL/well) can bind Anti-NKp46, hIgG4 with a linear range of 0.4-6 ng/mL. Please note that there may be a cross-reaction between anti-human IgG Fc antibodies and llama IgG Fc tag, also between anti-llama IgG Fc antibodies and human IgG Fc tag. |
Molecular Mass : |
This protein carries a llama IgG2b Fc tag at the C-terminus.The protein has a calculated MW of 54.7 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Proteinlength : |
Pro22-Arg257 |
Endotoxin : |
Less than 0.01 EU per μg by the LAL method. |
Purity : |
>90% as determined by SDS-PAGE. |
Storage : |
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |