Source : |
HEK293 |
Species : |
Cynomolgus/Rhesus macaque |
Tag : |
Fc |
Form : |
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : |
Immobilized Cynomolgus / Rhesus macaque CTLA-4, His Tag at 2 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque B7-1, Fc Tag with a linear range of 1-8 ng/mL. |
Molecular Mass : |
This protein carries a human IgG1 Fc tag at the C-terminus.The protein has a calculated MW of 50.5 kDa. The protein migrates as 60-90 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation. |
Protein Length : |
Val 35-Asn 242 |
Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
Purity : |
>95% as determined by SDS-PAGE. >90% as determined by SEC-MALS. |
Storage : |
For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |