Source : |
HEK293 |
Species : |
Cynomolgus / Rhesus macaque |
Tag : |
His-Avi |
Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : |
Immobilized Biotinylated Cynomolgus / Rhesus macaque OX40, His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Cynomolgus / Rhesus macaque OX40 Ligand, Mouse IgG2a Fc Tag with a linear range of 0.1-4 ng/mL. Immobilized Biotinylated Cynomolgus / Rhesus macaque OX40, His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Human OX40 Ligand, Fc Tag with a linear range of 0.1-1 ng/mL. |
Molecular Mass : |
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag).The protein has a calculated MW of 23.8 kDa. The protein migrates as 40-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Proteinlength : |
Lys28-Ala214 |
Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
Purity : |
>90% as determined by SDS-PAGE. |
Storage : |
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |