| Source : |
HEK293 |
| Species : |
Cynomolgus/Rhesus macaque |
| Tag : |
His |
| Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant. |
| Bio-activity : |
Immobilized Cynomolgus/Rhesus macaque OX40 Ligand, His Tag at 2 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque OX40, Fc Tag with a linear range of 0.1-3 ng/mL. Immobilized Cynomolgus/Rhesus macaque OX40 Ligand, His Tag at 2 μg/mL (100 μL/well) can bind Human OX40, Fc Tag with a linear range of 0.1-1 ng/mL. Immobilized Cynomolgus / Rhesus macaque OX40, Fc Tag at 5 μg/mL (100 μL/well) can bind Cynomolgus/Rhesus macaque OX40 Ligand, His Tag with a linear range of 20-78 ng/mL. |
| Molecular Mass : |
Molecular CharacterizationThis protein carries a polyhistidine tag at the N-terminusThe protein has a calculated MW of 49.3 kDa. The protein migrates as 65-80 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation. |
| Proteinlength : |
Gln51-Leu183 |
| Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
| Purity : |
>90% as determined by SDS-PAGE. >95% as determined by SEC-MALS. |
| Storage : |
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
