Active Recombinant EndoS enzyme
Cat.No. : | EndoS-10S |
Product Overview : | Recombinant enzyme with no detectable exooglycosidase or other endoglycosidases contaminating activities. |
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Description : | Endo S is an endoglycosidase specific for cleaving the N-linked glycans from the chitobiose core of the heavy chain of native IgG |
Tag : | Tagged with a chitin binding domain (CBD) for easy removal |
Bio-activity : | 5 μg of IgG in 1×GlycoBuffer 1 are incubated with two-fold dilutions of Endo S for 1 hour at 37 centigrade. Separation of reaction products is visualized by SDS-PAGE. |
Molecular Mass : | 136 kDa |
Purity : | ≥ 95 % purity, as determined by SDS-PAGE and intact ESI-MS |
Unit Definition : | One unit is defined as the amount of enzyme required to remove > 95 % of the carbohydrate from 5 μg of native mouse monoclonal IgG in 1 hour at 37 centigrade in a total reaction volume of 10 μL. |
Applications : | Glycerol-free for optimal performance in HPLC and mass spectrometry analysis |
Usage : | 1 × GlycoBuffer 1 (5 mM CaCl2, 50 mM sodium acetate, pH 5.5 at 25 centigrade), Incubate at 37 centigrade. |
Notes : | Removal of Endo S from thedeglycosylation reaction can be scaled uplinearly with larger volumes of chitin magneticbeads. The binding capacity of Chitin Magnetic Beads is 1 mg of CBD-tagged protein/mL of beads. |
Stability : | Optimal activity and stability for up to 12 months |
Storage : | Recommended storage temperature is 4 centigrade, avoid repeat freeze-thaw cycles. |
Storage Buffer : | 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH 7.5 at 25 centigrade. |
Official Symbol : | EndoS |
Synonyms : | EndoS |
Official Symbol : | EndoS |
Synonyms : | EndoS |
Products Types
◆ Recombinant Protein | ||
EndoS-1613S | Recombinant Streptococcus pyogenes EndoS Protein | +Inquiry |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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