Active Recombinant Entamoeba histolytica ADH1 protein, His-tagged

• Cat.No.: ADH1-156
• Product Overview: Recombinant Entamoeba histolytica ADH1 fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: Alcohol dehydrogenase with a preference for medium chain secondary alcohols, such as 2-butanol and isopropanol. Has very low activity with primary alcohols, such as ethanol. Under physiological conditions, the enzyme reduces aldehydes and 2-ketones to produce secondary alcohols. Is also active with acetaldehyde and propionaldehyde.
• Source: E. coli
• Species: Entamoeba histolytica
• Tag: His
• Form: 1 mg/mL solution in 50 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl and 50% glycerol
• Bio-activity: This NADP-dependent isopropanol dehydrogenase has biological activity ≥60U/mg.
• Molecular Mass: ~40.9 kDa
• Protein length: 360 amino acids
• Purity: Purity by SDS-PAGE: ≥95%
• Unit Definition: One unit is the amount of enzyme that will generate 1.0 µmole of NADPH per minute at pH 8 at 37 °C.
• Notes: Centrifuge the vial prior to opening
• Storage: Storage at -20 centigrade

Reviews

09/26/2021

Highly effective in blocking protein-protein interactions in competition assays.

11/14/2018

Shows excellent target engagement and modulation in drug discovery studies.

Q&As

How does Adh1 contribute to alcohol metabolism, and what is its catalytic efficiency and substrate specificity compared to other ADH isoforms?

Adh1 plays a significant role in alcohol metabolism, showing specific catalytic efficiency and substrate specificity for the conversion of alcohol to acetaldehyde.

How does the expression or activity of Adh1 change in response to pharmacological agents or environmental factors, and what are the implications for alcohol metabolism or therapy?

Pharmacological agents or environmental factors can modulate Adh1 expression or activity, potentially influencing alcohol metabolism and providing avenues for therapeutic interventions or personalized medicine.

What are the regulatory mechanisms controlling Adh1 expression, and how do they respond to alcohol exposure or other metabolic factors?

The expression of Adh1 is regulated by specific mechanisms, including transcriptional and post-transcriptional regulation, which can respond to alcohol exposure or metabolic factors such as hormonal signaling.

Are there any cofactors or coenzymes required for Adh1 activity, and how do they influence its catalytic function?

Adh1 requires specific cofactors or coenzymes, such as NAD+ or NADH, for its activity, serving as electron carriers and modulators of Adh1 function.

What are the consequences of Adh1 deficiency or knockout in experimental models, and how does it affect alcohol metabolism and related phenotypes?

Adh1 deficiency or knockout in experimental models leads to altered alcohol metabolism and related phenotypes, such as decreased alcohol clearance and increased sensitivity to alcohol-induced effects.

How does Adh1 expression or activity vary in different species or populations, and what are the underlying genetic and environmental factors influencing these variations?

Adh1 expression or activity may vary among different species or populations, influenced by genetic and environmental factors, contributing to inter-individual or inter-species differences in alcohol metabolism and tolerance.

What are the structural features of Adh1, and how do they relate to its enzymatic activity and substrate binding?

The structural features of Adh1, such as its active site and binding domains, are critical for its enzymatic activity and substrate recognition, enabling efficient alcohol oxidation.

Can Adh1 metabolize other substrates apart from alcohol, and what are the implications for its broader metabolic role?

Adh1 has the capacity to metabolize other substrates beyond alcohol, suggesting its involvement in broader metabolic pathways and potential metabolic interactions.

Are there any genetic variations or polymorphisms in the Adh1 gene associated with differences in alcohol metabolism or susceptibility to alcohol-related disorders?

Genetic variations or polymorphisms in the Adh1 gene have been associated with differences in alcohol metabolism and susceptibility to alcohol-related disorders, highlighting the impact of genotype on phenotype.

What is the tissue-specific expression pattern of Adh1, and how does it differ from other ADH isoforms in terms of its expression levels and distribution across organs and cell types?

Adh1 exhibits a tissue-specific expression pattern, with higher expression levels in certain organs such as the liver, and its distribution may differ from other ADH isoforms, reflecting functional specialization.