Active Recombinant Full Length Human ACP1 Protein, N-GST-tagged

• Cat.No.: ACP1-93HFL
• Product Overview: N-terminal GSTtagged, recombinant, human LMPTP-A, full length, expressed in E. coli cells. Purified using glutathione agarose.

Specification

• Description: LMPTP-A belongs to the phosphotryosine protein phosphatase family of proteins. It functions as an acid phosphatase and a protein tyrosine phosphatase by hydrolyzing protein tyrosine phosphate to protein tyrosine and orthophosphate. This enzyme also hydrolyzes orthophosphoric monoesters to alcohol and orthophosphate. This gene is genetically polymorphic, and three common alleles segregating at the corresponding locus give rise to six phenotypes. Each allele appears to encode at least two electrophoretically different isozymes, Bf and Bs, which are produced in allele-specific ratios. Three transcript variants encoding distinct isoforms have been identified for this gene.
• Source: E. coli
• Species: Human
• Tag: GST
• Protein length: Full Length
• Form: 2.075mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 10% glycerol, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 8243U/mg, where one unit of LMPTP-A activity is defined as the release of 1nmol of phosphate per minute from the phosphorylated substrate 6,8-difluoro-4-methylumbelliferyl phosphate (DiFMUP) at room temperature.
• Molecular Mass: 44.5 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSMAEQ ATKSVLFVCL 241 GNICRSPIAE AVFRKLVTDQ NISENWRVDS AATSGYEIGN PPDYRGQSCM KRHGIPMSHV 301 ARQITKEDFA TFDYILCMDE SNLRDLNRKS NRVKTCKAKI ELLGSYDPQK QLIIEDPYYG 361 NDSDFETVYQ QCVRCCRAFL EKAH
• Purity: Purity 94.1% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: ACP1 acid phosphatase 1 [ Homo sapiens (human) ]
• Official Symbol: ACP1
• Synonyms: ACP1; acid phosphatase 1; HAAP; LMWPTP; LMW-PTP
• Gene ID: 52
• mRNA Refseq: NM_004300.4
• Protein Refseq: NP_004291.1
• MIM: 171500
• UniProt ID: P24666

Reviews

02/17/2023

The answers are very concise.

02/16/2023

There are all the questions I want to know.

02/16/2023

The answer was very patient.

Q&As

What are the two subtypes of ACP1?

F and S.

How to analyze ACP1 polymorphism?

Through the polymerase chain reaction-resreiction fragment length polymorphism.

What disease would be influenced by ABHD14A?

Esophageal cancer, gastric cancer and gastrointestinal cancer will lead to up-regulation of ACP1 expression, while colorectal cancer will reduce it.

Why is the blood glucose level of inactive ACP1 A low?

Because ACP1 has low dephosphorylation effect on insulin receptor.

How ACP1 works on respiration and cell oxidation?

To adjust the level of FMN and flavin adenine dinucleotide.

How to detect the protein level of ACP1?

Immunohistochemical staining observation of two related protein sections.

How many codominant alleles are there in ACP1?

Three.

What activities may ACP1 participate in the brain?

The cholesterol biosynthesis, neutotransmitter synthesis and it links to behavioral and cellular response to stress via p190RhoGAP

What's the ACP1 blocking buffer？

The PBS conraining 10% goat serum.

What protein is significantly associated with ACP1 in the brain?

The TPH2, DDC and GAD1.