Active Recombinant Full Length Human ACTA2 Protein, C-Flag-tagged

• Cat.No.: ACTA2-676HFL
• Product Overview: Recombinant Full Length Human ACTA2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a smooth muscle actin that is involved in vascular contractility and blood pressure homeostasis. Mutations in this gene cause a variety of vascular diseases, such as thoracic aortic disease, coronary artery disease, stroke, and Moyamoya disease, as well as multisystemic smooth muscle dysfunction syndrome.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: Pull-down assay
• Molecular Mass: 41.8 kDa
• AA Sequence: MCEEEDSTALVCDNGSGLCKAGFAG DDAPRAVFPSIVGRPRHQGVMVGMG QKDSYVGDEAQSKRGILTLK YPIEHGIITNWDDMEKIWHHSFYNE LRVAPEEHPTLLTEAPLNPKANREK MTQIMFETFNVPAMYVAIQA VLSLYASGRTTGIVLDSGDGVTHNV PIYEGYALPHAIMRLDLAGRDLTDY LMKILTERGYSFVTTAEREI VRDIKEKLCYVALDFENEMATAASS SSLEKSYELPDGQVITIGNERFRCP ETLFQPSFIGMESAGIHETT YNSIMKCDIDIRKDLYANNVLSGGT TMYPGIADRMQKEITALAPSTMKIK IIAPPERKYSVWIGGSILAS
  LSTFQQMWISKQEYDEAGPSIVHRK CFTRTRPLEQKLISEEDLAANDILD YKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Vascular smooth muscle contraction

Gene Information

• Gene Name: ACTA2 actin alpha 2, smooth muscle [ Homo sapiens (human) ]
• Official Symbol: ACTA2
• Synonyms: ACTSA
• Gene ID: 59
• mRNA Refseq: NM_001141945.3
• Protein Refseq: NP_001135417.1
• MIM: 102620
• UniProt ID: P62736