Active Recombinant Full Length Human AURKB Protein, DDK-tagged, Biotinylated

• Cat.No.: AURKB-4230HF
• Product Overview: Full-length human AurB [1-344(end) amino acids of accession number Q96GD4] was co-expressed as N- terminal DYKDDDDK tagged, biotinylated protein (43 kDa) with His-tagged INCENP(INBOX) [803- 918(end) amino acids of accession number AAU04398] using baculovirus expression system. The protein was purified by using DYKDDDDK tag antibody agarose and activated with ATP. Activated protein was purified by using gel filtration.

Specification

• Source: Insect (sf21)
• Species: Human
• Tag: DDK
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device.
• Protein length: 1-344(end)
• Purity: 82%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws.

Gene Information

• Gene Name: aurora kinase B
• Official Symbol: AURKB
• Synonyms: AURKB;AIK2;AIM-1;AIM1;ARK-2;ARK2;AurB;IPL1;PPP1R48;STK-1;STK12;STK5;aurkb-sv1;aurkb-sv2;Aik2
• Protein Refseq: Q96GD4
• UniProt ID: Q96GD4

Reviews

12/28/2021

AURKB's exceptional repeatability allows for its reliable use in a range of applications, making it a top choice for researchers.

08/31/2021

Very pure and can be used with confidence.

02/03/2019

The quality is very high and very reliable.

Q&As

What are the interactions between AURKB protein and cell cycle regulatory proteins?

AURKB protein interacts with a variety of cell cycle regulatory proteins, including Cdk1, INCENP, Survivin, etc., to regulate the progression of cell mitosis.

What are the intracellular substrates of AURKB protein?

The substrates of AURKB include chromosome-associated proteins, cytoskeletal proteins and other cell cycle regulatory proteins. Phosphorylation modification of these substrates regulates chromosome segregation and cell division during mitosis.

What is the cellular localization of AURKB protein?

AURKB protein is mainly located in the nucleus and cytoplasm and participates in the regulation of cell mitosis.

What is the expression pattern of AURKB gene?

The expression of the AURKB gene is regulated by cell cycle regulators and other transcription factors and is mainly expressed in the mitotic phase of cells.

How does AURKB protein activity work?

The activity of AURKB protein is regulated by a variety of factors, including phosphorylation modification, protein interaction and cell cycle regulation.

At what stage of cell mitosis does AURKB protein reach its peak expression?

The protein reaches its peak expression during early and metaphase of cell mitosis and is closely associated with chromosome segregation and cell division.