Active Recombinant Full Length Human CSNK2A1 Protein, N-His-tagged

• Cat.No.: CSNK2A1-38HFL
• Product Overview: N-terminal 6His-tagged, recombinant, human, full length Casein Kinase 2 alpha subunit. Expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: Mammalian casein kinase 2α1 (CK2a1) is a highly conserved serine-threonine protein kinase that is constitutively expressed in all eukaryotic cell types including yeast, insects, and vertebrates. The enzyme can utilize GTP or ATP as a phosphate donor. Casein kinase activity is stimulated by mitogens in a cell type-specific manner during development and embryogenesis. Inhibition of CK2α1 may be useful in the treatment of human squamous cell carcinoma, adenocarcinoma of the lung, prostatic hyperplasia and lymphomas.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 1.26mg/ml of enzyme in 20mM MOPS pH7.4, 1mM EDTA, 50% glycerol, 1mM benzamidine, 0.1% Triton X100, 0.3% 2-mercaptoethanol. Liquid at - 20centigrade.
• Bio-activity: 1409U/mg, where one unit of Casein Kinase 2, active activity is defined as 1nmol phosphate incorporated into 165μM Casein Kinase 2 substrate peptide per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 48.7 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPMS GPVPSRARVY TDVNTHRPRE YWDYESHVVE 61 WGNQDDYQLV RKLGRGKYSE VFEAINITNN EKVVVKILKP VKKKKIKREI KILENLRGGP 121 NIITLADIVK DPVSRTPALV FEHVNNTDFK QLYQTLTDYD IRFYMYEILK ALDYCHSMGI 181 MHRDVKPHNV MIDHEHRKLR LIDWGLAEFY HPGQEYNVRV ASRYFKGPEL LVDYQMYDYS 241 LDMWSLGCML ASMIFRKEPF FHGHDNYDQL VRIAKVLGTE DLYDYIDKYN IELDPRFNDI 301 LGRHSRKRWE RFVHSENQHL VSPEALDFLD KLLRYDHQSR LTAREAMEHP YFYTVVKDQA 361 RMGSSSMPGG STPVSSANMM SGISSVPTPS PLGPLAGSPV IAAANPLGMP VPAAAGAQQ
• Purity: Purity 96.5% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: CSNK2A1 casein kinase 2 alpha 1 [ Homo sapiens (human) ]
• Official Symbol: CSNK2A1
• Synonyms: CSNK2A1; casein kinase 2 alpha 1; CKII; Cka1; Cka2; CK2A1; OCNDS
• Gene ID: 1457
• mRNA Refseq: NM_001362770.2
• Protein Refseq: NP_001349699.1
• MIM: 115440
• UniProt ID: P68400