Active Recombinant Full Length Human EEF1G Protein, C-Flag-tagged

• Cat.No.: EEF1G-566HFL
• Product Overview: Recombinant Full Length Human EEF1G Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: Biolayer interferometry (BLI) assay
• Molecular Mass: 49.9 kDa
• AA Sequence: MAAGTLYTYPENWRAFKALIAAQYS GAQVRVLSAPPHFHFGQTNRTPEFL RKFPAGKVPAFEGDDGFCVF ESNAIAYYVSNEELRGSTPEAAAQV VQWVSFADSDIVPPASTWVFPTLGI MHHNKQATENAKEEVRRILG LLDAYLKTRTFLVGERVTLADITVV CTLLWLYKQVLEPSFRQAFPNTNRW FLTCINQPQFRAVLGEVKLC EKMAQFDAKKFAETQPKKDTPRKEK GSREEKQKPQAERKEEKKAAAPAPE EEMDECEQALAAEPKAKDPF AHLPKSTFVLDEFKRKYSNEDTLSV ALPYFWEHFDKDGWSLWYSEYRFPE ELTQTFMSCNLITGMFQRLD KLRKNAFASVILFGTNNSSSISGVW VFRGQELAFPLSPDWQVDYESYTWR KLDPGSEETQTLVREYFSWE
  GAFQHVGKAFNQGKIFKTRTRPLEQ KLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: EEF1G eukaryotic translation elongation factor 1 gamma [ Homo sapiens (human) ]
• Official Symbol: EEF1G
• Synonyms: EF1G; GIG35
• Gene ID: 1937
• mRNA Refseq: NM_001404.5
• Protein Refseq: NP_001395.1
• MIM: 130593
• UniProt ID: P26641