| Description : |
This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. This gene produces multiple isoforms by using alternative translational start codons and by alternative splicing. Related pseudogenes have been identified on chromosomes 7 and 14. |
| Source : |
Sf9 cells |
| Species : |
Human |
| Tag : |
N-GST/6×His |
| Molecular Mass : |
73163 Da |
| Protein Length : |
M1-P428 |
| AA Sequence : |
MSPILGYWKIKGLVQPTRLLLEYLE EKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYIDGDVKLTQSMAIIRYI ADKHNMLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKVDFLSK LPEMLKMFEDRLCHKTYLNGDHVTH PDFMLYDALDVVLYMDPMCLDAFPK LVCFKKRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHPPKSDPMGHH HHHHGRDSLEVLFQGPLAMDPSVTL WQFLLQLLREQGNGHIISWTSRDGG EFKLVDAEEVARLWGLRKNKTNMNY DKLSRALRYYYDKNIIRKVSGQKFV YKFVSYPEVAGCSTEDCPPQPEVSV TSTMPNVAPAAIHAAPGDTVSGKPG TPKGAGMAGPGGLARSSRNEYMRSG LYSTFTIQSLQPQPPPHPRPAVVLP SAAPAGAAAPPSGSRSTSPSPLEAC LEAEEAGLPLQVILTPPEAPNLKSE ELNVEPGLGRALPPEVKVEGPKEEL EVAGERGFVPETTKAEPEVPPQEGV PARLPAVVMDTAGQAGGHAASSPEI SQPQKGRKPRDLELPLSPSLLGGPG PERTPGSGSGSGLQAPGPALTPSLL PTHTLTPVLLTPSSLPPSIHFWSTL SPIAPRSPAKLSFQFPSSGSAQVHI PSISVDGLSTPVVLSPGPQKP |
| Applications : |
ELK1 has been validated for use as substrate in radiometric in-vitro kinase activity assays. |
| Storage : |
At -80 centigrade
For complete recovery, mix well and spin before use. Product must not be stored in diluted solutions, aliquots below 10μl are not advisable. Avoid repeated freeze-thaw cycles! |
| Concentration : |
0.303 μg/μL (Bradford method using BSA as standard protein) |
| Storage Buffer : |
50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20 % glycerol |
