Active Recombinant Full Length Human EZH2 Protein, C-Flag-tagged

• Cat.No.: EZH2-65HFL
• Product Overview: Recombinant Full Length Human EZH2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: EMSA assay
  Binding assay (AlphaScreen)
  In vitro ubiquitination assay substrate
• Molecular Mass: 85.8 kDa
• AA Sequence: MGQTGKKSEKGPVCWRKRVKSEYMR LRQLKRFRRADEVKSMFSSNRQKIL ERTEILNQEWKQRRIQPVHI LTSVSSLRGTRECSVTSDLDFPTQV IPLKTLNAVASVPIMYSWSPLQQNF MVEDETVLHNIPYMGDEVLD QDGTFIEELIKNYDGKVHGDRECGF INDEIFVELVNALGQYNDDDDDDDG DDPEEREEKQKDLEDHRDDK ESRPPRKFPSDKIFEAISSMFPDKG TAEELKEKYKELTEQQLPGALPPEC TPNIDGPNAKSVQREQSLHS FHTLFCRRCFKYDCFLHRKCNYSFH ATPNTYKRKNTETALDNKPCGPQCY QHLEGAKEFAAALTAERIKT PPKRPGGRRRGRLPNNSSRPSTPTI NVLESKDTDSDREAGTETGGENNDK EEEEKKDETSSSSEANSRCQ TPIKMKPNIEPPENVEWSGAEASMF RVLIGTYYDNFCAIARLIGTKTCRQ VYEFRVKESSIIAPAPAEDV DTPPRKKKRKHRLWAAHCRKIQLKK DGSSNHVYNYQPCDHPRQPCDSSCP CVIAQNFCEKFCQCSSECQN RFPGCRCKAQCNTKQCPCYLAVREC DPDLCLTCGAADHWDSKNVSCKNCS IQRGSKKHLLLAPSDVAGWG IFIKDPVQKNEFISEYCGEIISQDE ADRRGKVYDKYMCSFLFNLNNDFVV DATRKGNKIRFANHSVNPNC
  YAKVMMVNGDHRIGIFAKRAIQTGE ELFFDYRYSQADALKYVGIEREMEI PTRTRPLEQKLISEEDLAANDILDY KDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Transcription Factors

Gene Information

• Gene Name: EZH2 enhancer of zeste 2 polycomb repressive complex 2 subunit [ Homo sapiens (human) ]
• Official Symbol: EZH2
• Synonyms: WVS; ENX1; KMT6; WVS2; ENX-1; EZH2b; KMT6A
• Gene ID: 2146
• mRNA Refseq: NM_004456.5
• Protein Refseq: NP_004447.2
• MIM: 601573
• UniProt ID: Q15910