Active Recombinant Full Length Human F2 Protein, C-Flag-tagged

• Cat.No.: F2-490HFL
• Product Overview: Recombinant Full Length Human F2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes the prothrombin protein (also known as coagulation factor II). This protein is proteolytically cleaved in multiple steps to form the activated serine protease thrombin. The activated thrombin enzyme plays an important role in thrombosis and hemostasis by converting fibrinogen to fibrin during blood clot formation, by stimulating platelet aggregation, and by activating additional coagulation factors. Thrombin also plays a role in cell proliferation, tissue repair, and angiogenesis as well as maintaining vascular integrity during development and postnatal life. Peptides derived from the C-terminus of this protein have antimicrobial activity against E. coli and P. aeruginosa. Mutations in this gene lead to various forms of thrombosis and dysprothrombinemia. Rapid increases in cytokine levels following coronavirus infections can dysregulate the coagulation cascade and produce thrombosis, compromised blood supply, and organ failure.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: MS digestion standard
• Molecular Mass: 67.5 kDa
• AA Sequence: MAHVRGLQLPGCLALAALCSLVHSQ HVFLAPQQARSLLQRVRRANTFLEE VRKGNLERECVEETCSYEEA FEALESSTATDVFWAKYTACETART PRDKLAACLEGNCAEGLGTNYRGHV NITRSGIECQLWRSRYPHKP EINSTTHPGADLQENFCRNPDSSTT GPWCYTTDPTVRRQECSIPVCGQDQ VTVAMTPRSEGSSVNLSPPL EQCVPDRGQQYQGRLAVTTHGLPCL AWASAQAKALSKHQDFNSAVQLVEN FCRNPDGDEEGAWCYVAGKP GDFGYCDLNYCEEAVEEETGDGLDE DSDRAIEGRTATSEYQTFFNPRTFG SGEADCGLRPLFEKKSLEDK TERELLESYIDGRIVEGSDAEIGMS PWQVMLFRKSPQELLCGASLISDRW VLTAAHCLLYPPWDKNFTEN DLLVRIGKHSRTRYERNIEKISMLE KIYIHPRYNWRENLDRDIALMKLKK PVAFSDYIHPVCLPDRETAA SLLQAGYKGRVTGWGNLKETWTANV GKGQPSVLQVVNLPIVERPVCKDST RIRITDNMFCAGYKPDEGKR
  GDACEGDSGGPFVMKSPFNNRWYQM GIVSWGEGCDRDGKYGFYTHVFRLK KWIQKVIDQFGETRTRPLEQKLISE EDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protease, Secreted Protein
• Protein Pathways: Complement and coagulation cascades, Neuroactive ligand-receptor interaction, Regulation of actin cytoskeleton

Gene Information

• Gene Name: F2 coagulation factor II, thrombin [ Homo sapiens (human) ]
• Official Symbol: F2
• Synonyms: PT; THPH1; RPRGL2
• Gene ID: 2147
• mRNA Refseq: NM_000506.5
• Protein Refseq: NP_000497.1
• MIM: 176930
• UniProt ID: P00734