Active Recombinant Full Length Human FUBP1 Protein, C-Flag-tagged

• Cat.No.: FUBP1-166HFL
• Product Overview: Recombinant Full Length Human FUBP1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a single stranded DNA-binding protein that binds to multiple DNA elements, including the far upstream element (FUSE) located upstream of c-myc. Binding to FUSE occurs on the non-coding strand, and is important to the regulation of c-myc in undifferentiated cells. This protein contains three domains, an amphipathic helix N-terminal domain, a DNA-binding central domain, and a C-terminal transactivation domain that contains three tyrosine-rich motifs. The N-terminal domain is thought to repress the activity of the C-terminal domain. This protein is also thought to bind RNA, and contains 3'-5' helicase activity with in vitro activity on both DNA-DNA and RNA-RNA duplexes. Aberrant expression of this gene has been found in malignant tissues, and this gene is important to neural system and lung development. Binding of this protein to viral RNA is thought to play a role in several viral diseases, including hepatitis C and hand, foot and mouth disease. Alternative splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: EMSA assay
• Molecular Mass: 67.4 kDa
• AA Sequence: MADYSTVPPPSSGSAGGGGGGGGGG GVNDAFKDALQRARQIAAKIGGDAG TSLNSNDYGYGGQKRPLEDG DQPDAKKVAPQNDSFGTQLPPMHQQ QSRSVMTEEYKVPDGMVGFIIGRGG EQISRIQQESGCKIQIAPDS GGLPERSCMLTGTPESVQSAKRLLD QIVEKGRPAPGFHHGDGPGNAVQEI MIPASKAGLVIGKGGETIKQ LQERAGVKMVMIQDGPQNTGADKPL RITGDPYKVQQAKEMVLELIRDQGG FREVRNEYGSRIGGNEGIDV PIPRFAVGIVIGRNGEMIKKIQNDA GVRIQFKPDDGTTPERIAQITGPPD RCQHAAEIITDLLRSVQAGN PGGPGPGGRGRGRGQGNWNMGPPGG LQEFNFIVPTGKTGLIIGKGGETIK SISQQSGARIELQRNPPPNA DPNMKLFTIRGTPQQIDYARQLIEE KIGGPVNPLGPPVPHGPHGVPGPHG PPGPPGPGTPMGPYNPAPYN PGPPGPAPHGPPAPYAPQGWGNAYP HWQQQAPPDPAKAGTDPNSAAWAAY YAHYYQQQAQPPPAAPAGAP TTTQTNGQGDQQNPAPAGQVDYTKA WEEYYKKMGQAVPAPTGAPPGGQPD YSAAWAEYYRQQAAYYAQTS
  PQGMPQHPPAPQGQTRTRPLEQKLI SEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Stem cell - Pluripotency, Transcription Factors

Gene Information

• Gene Name: FUBP1 far upstream element binding protein 1 [ Homo sapiens (human) ]
• Official Symbol: FUBP1
• Synonyms: FBP; FUBP; hDH V
• Gene ID: 8880
• mRNA Refseq: NM_003902.5
• Protein Refseq: NP_003893.2
• MIM: 603444
• UniProt ID: Q96AE4