| Description : |
This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist. |
| Source : |
Mammalian cells |
| Species : |
Human |
| Tag : |
Flag |
| Form : |
25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol. |
| Bio-activity : |
FYN activity verified in a biochemical assay: FYN (FYN oncogene related to SRC, FGR, YES) activity was measured in a homogeneous time-resolved fluorescent (HTRF®) assay. FYN is a non-receptor tyrosine kinase and is a member of the Src kinase family consisting of Src, Fyn, Yes, Lck, Lyn, Hck, Fgr and Blk. Varying concentrations of FYN were added to a reaction mix containing ATP and a biotinylated kinase substrate and the reaction mixture was incubated to allow the protein to phosphorylate the substrate. HTRF detection reagents were then added, and the time-resolved fluorescent signal was measured on a Flexstation 3 microplate reader. The time resolved fluorescent signal is expressed as “delta R” or “&DeltaR” and is a ratio calculated from the fluorescent emission intensities of the donor and acceptor fluors. |
| Molecular Mass : |
60.6 kDa |
| AA Sequence : |
MGCVQCKDKEATKLTEERDGSLNQS SGYRYGTDPTPQHYPSFGVTSIPNY NNFHAAGGQGLTVFGGVNSS SHTGTLRTRGGTGVTLFVALYDYEA RTEDDLSFHKGEKFQILNSSEGDWW EARSLTTGETGYIPSNYVAP VDSIQAEEWYFGKLGRKDAERQLLS FGNPRGTFLIRESETTKGAYSLSIR DWDDMKGDHVKHYKIRKLDN GGYYITTRAQFETLQQLVQHYSERA AGLCCRLVVPCHKGMPRLTDLSVKT KDVWEIPRESLQLIKRLGNG QFGEVWMGTWNGNTKVAIKTLKPGT MSPESFLEEAQIMKKLKHDKLVQLY AVVSEEPIYIVTEYMNKGSL LDFLKDGEGRALKLPNLVDMAAQVA AGMAYIERMNYIHRDLRSANILVGN GLICKIADFGLARLIEDNEY TARQGAKFPIKWTAPEAALYGRFTI KSDVWSFGILLTELVTKGRVPYPGM NNREVLEQVERGYRMPCPQD
CPISLHELMIHCWKKDPEERPTFEY LQSFLEDYFTATEPQYQPGENLTRT RPLEQKLISEEDLAANDILDYKDDD DKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining. |
| Stability : |
Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Storage : |
Store at -80 centigrade. |
| Concentration : |
>50 ug/mL as determined by microplate BCA method. |
| Preparation : |
Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Protein Families : |
Druggable Genome, Protein Kinase |
| Protein Pathways : |
Adherens junction, Axon guidance, Fc epsilon RI signaling pathway, Focal adhesion, Natural killer cell mediated cytotoxicity, Pathogenic Escherichia coli infection, Prion diseases, T cell receptor signaling pathway, Viral myocarditis |
