Active Recombinant Full Length Human GEMIN5 Protein, C-Flag-tagged

• Cat.No.: GEMIN5-367HFL
• Product Overview: Recombinant Full Length Human GEMIN5 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a WD repeat protein that is a component of the survival of motor neurons (SMN) complex. The SMN complex plays a critical role in mRNA splicing through the assembly of spliceosomal small nuclear ribonucleoproteins (snRNPs), and may also mediate the assembly and transport of other classes of ribonucleoproteins. The encoded protein is the snRNA-binding component of the SMN complex. Dysregulation of this gene may play a role in alternative mRNA splicing and tumor cell motility. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: Pull-down assay
• Molecular Mass: 168.4 kDa
• AA Sequence: MGQEPRTLPPSPNWYCARCSDAVPG GLFGFAARTSVFLVRVGPGAGESPG TPPFRVIGELVGHTERVSGF TFSHHPGQYNLCATSSDDGTVKIWD VETKTVVTEHALHQHTISTLHWSPR VKDLIVSGDEKGVVFCYWFN RNDSQHLFIEPRTIFCLTCSPHHED LVAIGYKDGIVVIIDISKKGEVIHR LRGHDDEIHSIAWCPLPGED CLSINQEETSEEAEITNGNAVAQAP VTKGCYLATGSKDQTIRIWSCSRGR GVMILKLPFLKRRGGGIDPT VKERLWLTLHWPSNQPTQLVSSCFG GELLQWDLTQSWRRKYTLFSASSEG QNHSRIVFNLCPLQTEDDKQ LLLSTSMDRDVKCWDIATLECSWTL PSLGGFAYSLAFSSVDIGSLAIGVG DGMIRVWNTLSIKNNYDVKN FWQGVKSKVTALCWHPTKEGCLAFG TDDGKVGLYDTYSNKPPQISSTYHK KTVYTLAWGPPVPPMSLGGE GDRPSLALYSCGGEGIVLQHNPWKL SGEAFDINKLIRDTNSIKYKLPVHT EISWKADGKIMALGNEDGSI EIFQIPNLKLICTIQQHHKLVNTIS WHHEHGSQPELSYLMASGSNNAVIY VHNLKTVIESSPESPVTITE PYRTLSGHTAKITSVAWSPHHDGRL VSASYDGTAQVWDALREEPLCNFRG HRGRLLCVAWSPLDPDCIYS GADDFCVHKWLTSMQDHSRPPQGKK SIELEKKRLSQPKAKPKKKKKPTLR TPVKLESIDGNEEESMKENS GPVENGVSDQEGEEQAREPELPCGL APAVSREPVICTPVSSGFEKSKVTI NNKVILLKKEPPKEKPETLI KKRKARSLLPLSTSLDHRSKEELHQ DCLVLATAKHSRELNEDVSADVEER FHLGLFTDRATLYRMIDIEG KGHLENGHPELFHQLMLWKGDLKGV LQTAAERGELTDNLVAMAPAAGYHV WLWAVEAFAKQLCFQDQYVK AASHLLSIHKVYEAVELLKSNHFYR EAIAIAKARLRPEDPVLKDLYLSWG TVLERDGHYAVAAKCYLGAT CAYDAAKVLAKKGDAASLRTAAELA AIVGEDELSASLALRCAQELLLANN WVGAQEALQLHESLQGQRLV FCLLELLSRHLEEKQLSEGKSSSSY HTWNTGTEGPFVERVTAVWKSIFSL DTPEQYQEAFQKLQNIKYPS ATNNTPAKQLLLHICHDLTLAVLSQ QMASWDEAVQALLRAVVRSYDSGSF TIMQEVYSAFLPDGCDHLRD KLGDHQSPATPAFKSLEAFFLYGRL YEFWWSLSRPCPNSSVWVRAGHRTL SVEPSQQLDTASTEETDPET SQPEPNRPSELDLRLTEEGERMLST FKELFSEKHASLQNSQRTVAEVQET LAEMIRQHQKSQLCKSTANG PDKNEPEVEAEQPLCSSQSQCKEEK NEPLSLPELTKRLTEANQRMAKFPE SIKAWPFPDVLECCLVLLLI
  RSHFPGCLAQEMQQQAQELLQKYGN TKTYRRHCQTFCMTRTRPLEQKLIS EEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome

Gene Information

• Gene Name: GEMIN5 gem nuclear organelle associated protein 5 [ Homo sapiens (human) ]
• Official Symbol: GEMIN5
• Synonyms: NEDCAM; GEMIN-5
• Gene ID: 25929
• mRNA Refseq: NM_015465.5
• Protein Refseq: NP_056280.2
• MIM: 607005
• UniProt ID: Q8TEQ6