Active Recombinant Full Length Human GPNMB Protein, C-Flag-tagged

• Cat.No.: GPNMB-321HFL
• Product Overview: Recombinant Full Length Human GPNMB Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: Pull-down assay
• Molecular Mass: 61.5 kDa
• AA Sequence: MECLYYFLGFLLLAARLPLDAAKRF HDVLGNERPSAYMREHNQLNGWSSD ENDWNEKLYPVWKRGDMRWK NSWKGGRVQAVLTSDSPALVGSNIT FAVNLIFPRCQKEDANGNIVYEKNC RNEAGLSADPYVYNWTAWSE DSDGENGTGQSHHNVFPDGKPFPHH PGWRRWNFIYVFHTLGQYFQKLGRC SVRVSVNTANVTLGPQLMEV TVYRRHGRAYVPIAQVKDVYVVTDQ IPVFVTMFQKNDRNSSDETFLKDLP IMFDVLIHDPSHFLNYSTIN YKWSFGDNTGLFVSTNHTVNHTYVL NGTFSLNLTVKAAAPGPCPPPPPPP RPSKPTPSLATTLKSYDSNT PGPAGDNPLELSRIPDENCQINRYG HFQATITIVEGILEVNIIQMTDVLM PVPWPESSLIDFVVTCQGSI PTEVCTIISDPTCEITQNTVCSPVD VDEMCLLTVRRTFNGSGTYCVNLTL GDDTSLALTSTLISVPDRDP ASPLRMANSALISVGCLAIFVTVIS LLVYKKHKEYNPIENSPGNVVRSKG LSVFLNRAKAVFFPGNQEKD
  PLLKNQEFKGVSTRTRPLEQKLISE EDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Transmembrane

Gene Information

• Gene Name: GPNMB glycoprotein nmb [ Homo sapiens (human) ]
• Official Symbol: GPNMB
• Synonyms: NMB; HGFIN; PLCA3
• Gene ID: 10457
• mRNA Refseq: NM_001005340.2
• Protein Refseq: NP_001005340.1
• MIM: 604368
• UniProt ID: Q14956

Reviews

06/29/2022

The potency and power of this protein met my expectations.

02/01/2022

There are no adverse reactions and few side effects.

08/08/2021

The quality of this one is very good, and they also have a long lifespan, and I am very satisfied with this product.

Q&As

How to prepare GPNMB recombinant protein in the experiment?

In the experiment, the GPNMB gene can be introduced into the expression vector by gene cloning technology, and then the GPNMB recombinant protein can be expressed in Escherichia coli or eukaryotic cells.

How to use animal models to study the function and disease mechanism of GPNMB recombinant protein?

Animal models carrying human mutant genes can be constructed using transgenic or gene editing technology to study the role and disease mechanism of GPNMB recombinant protein in vivo. These animal models can be used to study the effects of GPNMB recombinant proteins on tumor growth, metastasis, and drug resistance.

What are the treatments for GPNMB recombinant protein?

The current treatments for GPNMB recombinant protein mainly include gene therapy, protein replacement therapy, and drug therapy for cell surface receptors and signal transduction processes.

What is the significance of GPNMB recombinant protein in tumor diagnosis and treatment?

By detecting the expression level and modification status of GPNMB recombinant protein, it can be used to diagnose certain tumor diseases. In addition, the recombinant protein of GPNMB may also become a new target for the treatment of tumors, providing new ideas and methods for tumor treatment.

How does GPNMB recombinant protein interact with other proteins?

GPNMB recombinant protein can interact with other proteins, such as cell surface receptors such as integrin, and participate in cell signal transduction processes.

If the level of GPNMB recombinant protein is abnormal, what should be treated?

If the level of GPNMB recombinant protein is abnormal, the treatment may include the use of drugs or other special treatments, depending on the condition and the doctor's recommendation.