Active Recombinant Full Length Human GRIA1 Protein, C-Flag-tagged

• Cat.No.: GRIA1-236HFL
• Product Overview: Recombinant Full Length Human GRIA1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: In vitro ubiquitination assay substrate
• Molecular Mass: 101.3 kDa
• AA Sequence: MQHIFAFFCTGFLGAVVGANFPNNI QIGGLFPNQQSQEHAAFRFALSQLT EPPKLLPQIDIVNISDSFEM TYRFCSQFSKGVYAIFGFYERRTVN MLTSFCGALHVCFITPSFPVDTSNQ FVLQLRPELQDALISIIDHY KWQKFVYIYDADRGLSVLQKVLDTA AEKNWQVTAVNILTTTEEGYRMLFQ DLEKKKERLVVVDCESERLN AILGQIIKLEKNGIGYHYILANLGF MDIDLNKFKESGANVTGFQLVNYTD TIPAKIMQQWKNSDARDHTR VDWKRPKYTSALTYDGVKVMAEAFQ SLRRQRIDISRRGNAGDCLANPAVP WGQGIDIQRALQQVRFEGLT GNVQFNEKGRRTNYTLHVIEMKHDG IRKIGYWNEDDKFVPAATDAQAGGD NSSVQNRTYIVTTILEDPYV MLKKNANQFEGNDRYEGYCVELAAE IAKHVGYSYRLEIVSDGKYGARDPD TKAWNGMVGELVYGRADVAV APLTITLVREEVIDFSKPFMSLGIS IMIKKPQKSKPGVFSFLDPLAYEIW MCIVFAYIGVSVVLFLVSRF SPYEWHSEEFEEGRDQTTSDQSNEF GIFNSLWFSLGAFMQQGCDISPRSL SGRIVGGVWWFFTLIIISSY TANLAAFLTVERMVSPIESAEDLAK QTEIAYGTLEAGSTKEFFRRSKIAV FEKMWTYMKSAEPSVFVRTT EEGMIRVRKSKGKYAYLLESTMNEY IEQRKPCDTMKVGGNLDSKGYGIAT PKGSALRGPVNLAVLKLSEQ GVLDKLKSKWWYDKGECGSKDSGSK DKTSALSLSNVAGVFYILIGGLGLA MLVALIEFCYKSRSESKRMK GFCLIPQQSINEAIRTSTLPRNSGA GASSGGSGENGRVVSHDFPKSMQSI PCMSHSSGMPLGATGLTRTRPLEQK LISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Long-term depression, Long-term potentiation, Neuroactive ligand-receptor interaction

Gene Information

• Gene Name: GRIA1 glutamate ionotropic receptor AMPA type subunit 1 [ Homo sapiens (human) ]
• Official Symbol: GRIA1
• Synonyms: GLUH1; GLUR1; GLURA; GluA1; HBGR1; MRD67; MRT76
• Gene ID: 2890
• mRNA Refseq: NM_001114183.2
• Protein Refseq: NP_001107655.1
• MIM: 138248
• UniProt ID: P42261