Active Recombinant Full Length Human GRK4 Protein

• Cat.No.: GRK4-4746HF
• Product Overview: Full-length human GPRK4 [1-578(end) amino acids of accession number NP_892027.2] was expressed as N-terminal GST-fusion protein using baculovirus expression system. GST-GPRK4 was purified by using glutathione sepharose chromatography. GST-GPRK4 was cleaved by PreScission protease and GST-free GPRK4(67 kDa) was collected as flow-through fraction from glutathione sepharose chromatography.

Specification

• Source: Insect (sf21)
• Species: Human
• Tag: Tag-free
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
• Protein length: 1-578
• Purity: 78%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws.

Gene Information

• Gene Name: G protein-coupled receptor kinase 4
• Official Symbol: GRK4
• Synonyms: GRK4;GPRK2L;GPRK4;GRK4a;IT11
• Protein Refseq: NP_892027.2
• UniProt ID: P32298