Active Recombinant Full Length Human GRK4 Protein, C-His-tagged

• Cat.No.: GRK4-74HFL
• Product Overview: C-terminal 6His-tagged, recombinant, human GRK4 full length expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.

Specification

• Description: GRK4 (G protein-coupled receptor kinase 4) is a serine/threonine protein kinase that belongs to the GRK family of kinases. It is primarily expressed in the kidney, where it plays a role in the regulation of blood pressure and sodium homeostasis. GRK4 has been implicated in the development of hypertension and renal disease, as its upregulation or over activation can lead to an increase in blood pressure and sodium reabsorption. Research has also suggested that GRK4 may play a role in the pathogenesis of several other diseases, including heart failure and obesity. In heart failure, GRK4 has been shown to be upregulated, and may contribute to the development of cardiac dysfunction. In obesity, GRK4 has been implicated in the regulation of adipocyte differentiation and metabolism, and may play a role in the development of insulin resistance.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 0.71 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 89 U/mg, where one unit of GRK4, active activity is defined as 1 nmol phosphate incorporated into 1.25 mg/ml casein per minute at 30 centigrade with a final ATP concentration of 100 μM.
• Molecular Mass: 67.7 kDa
• Purity: 64% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: GRK4 G protein-coupled receptor kinase 4 [ Homo sapiens (human) ]
• Official Symbol: GRK4
• Synonyms: GRK4; G protein-coupled receptor kinase 4; IT11; GPRK4; GRK4a; GPRK2L
• Gene ID: 2868
• mRNA Refseq: NM_182982.3
• Protein Refseq: NP_892027.2
• MIM: 137026
• UniProt ID: P32298