Active Recombinant Full Length Human GRK6 Protein, N-His-tagged

• Cat.No.: GRK6-76HFL
• Product Overview: N-terminal 6Histagged, recombinant, full length human GRK6 expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: GRK6 is a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. Several transcript variants encoding different isoforms have been described for this gene.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 1.323mg/ml of enzyme in 50mM Tris/HCl pH8.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 62U/mg, where one unit of GRK6, active activity is defined as 1nmol phosphate incorporated into 2mg/ml casein per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 69.8 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF MELENIVANT VLLKAREGGG GNRKGKSKKW 61 RQMLQFPHIS QCEELRLSLE RDYHSLCERQ PIGRLLFREF CATRPELSRC VAFLDGVAEY 121 EVTPDDKRKA CGRQLTQNFL SHTGPDLIPE VPRQLVTNCT QRLEQGPCKD LFQELTRLTH 181 EYLSVAPFAD YLDSIYFNRF LQWKWLERQP VTKNTFRQYR VLGKGGFGEV CACQVRATGK 241 MYACKKLEKK RIKKRKGEAM ALNEKQILEK VNSRFVVSLA YAYETKDALC LVLTLMNGGD 301 LKFHIYHMGQ AGFPEARAVF YAAEICCGLE DLHRERIVYR DLKPENILLD DHGHIRISDL 361 GLAVHVPEGQ TIKGRVGTVG YMAPEVVKNE RYTFSPDWWA LGCLLYEMIA GQSPFQQRKK 421 KIKREEVERL VKEVPEEYSE RFSPQARSLC SQLLCKDPAE RLGCRGGSAR EVKEHPLFKK 481 LNFKRLGAGM LEPPFKPDPQ AIYCKDVLDI EQFSTVKGVE LEPTDQDFYQ KFATGSVPIP 541 WQNEMVETEC FQELNVFGLD GSVPPDLDWK GQPPAPPKKG LLQRLFSRQD CCGNCSDSEE 601 ELPTRL
• Purity: Purity 94.9% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: GRK6 G protein-coupled receptor kinase 6 [ Homo sapiens (human) ]
• Official Symbol: GRK6
• Synonyms: GRK6; G protein-coupled receptor kinase 6; GPRK6
• Gene ID: 2870
• mRNA Refseq: NM_002082.4
• Protein Refseq: NP_002073.2
• MIM: 600869
• UniProt ID: P43250