| Description : |
HIPK4 (Homeodomain Interacting Protein Kinase 4) is a serine/threonine protein kinase that belongs to the HIPK (Homeodomain Interacting Protein Kinase) family of kinases. It is involved in the regulation of a variety of cellular processes, including transcriptional regulation, cell proliferation, and apoptosis. Research has suggested that HIPK4 plays a role in several diseases, including cancer and developmental disorders. In cancer, HIPK4 has been shown to promote tumor cell survival and proliferation, and alterations in HIPK4 expression and activity have been observed in various types of cancer. Additionally, HIPK4 has been implicated in the regulation of neural development and has been linked to developmental disorders such as autism spectrum disorder. HIPK4 has also been suggested as a potential therapeutic target in cancer, and efforts to develop small-molecule inhibitors of the kinase are underway. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
His |
| Protein length : |
Full Length |
| Form : |
0.53 mg/ml of enzyme in 50 mM Tris/HCl pH7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
858 U/mg, where one unit of HIPK4 activity is defined as 1 nmol phosphate incorporated into 250 μM RRRFRPASPLRGPPK per minute at 30 centigrade with a final ATP concentration of 100 μM. |
| Molecular Mass : |
71 kDa |
| AA Sequence : |
1 MSTIQSETDC YDIIEVLGKG TFGEVAKGWR RSTGEMVAIK ILKNDAYRNR IIKNELKLLH 61 CMRGLDPEEA HVIRFLEFFH DALKFYLVFE LLEQNLFEFQ KENNFAPLPA RHIRTVTLQV 121 LTALARLKEL AIIHADLKPE NIMLVDQTRC PFRVKVIDFG SASIFSEVRY VKEPYIQSRF 181 YRAPEILLGL PFCEKVDVWS LGCVMAELHL GWPLYPGNNE YDQVRYICET QGLPKPHLLH 241 AACKAHHFFK RNPHPDAANP WQLKSSADYL AETKVRPLER RKYMLKSLDQ IETVNGGSVA 301 SRLTFPDREA LAEHADLKSM VELIKRMLTW ESHERISPSA ALRHPFVSMQ QLRSAHETTH 361 YYQLSLRSYR LSLQVEGKPP TPVVAAEDGT PYYCLAEEKE AAGMGSVAGS SPFFREEKAP 421 GMQRAIDQLD DLSLQEAGHG LWGETCTNAV SDMMVPLKAA ITGHHVPDSG PEPILAFYSS 481 RLAGRHKARK PPAGSKSDSN FSNLIRLSQV SPEDDRPCRG SSWEEGEHLG ASAEPLAILQ 541 RDEDGPNIDN MTMEAERPDP ELFDPSSCPG EWLSEPDCTL ESVRGPRAQG LPPRRSHQHG 601 PPRGATSFLQ HVTGHHGPGP GHHHHHH |
| Purity : |
Purity 71% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
1 year at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
